tris buffer solution Search Results


chondroitinase abc solution  (AMS Biotechnology)
96
AMS Biotechnology chondroitinase abc solution
Chondroitinase Abc Solution, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris hcl  (Chem Impex International)
95
Chem Impex International tris hcl
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Hcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris glycine transfer buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tris glycine transfer buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Glycine Transfer Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris ethylenediaminetetraacetic acid  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology tris ethylenediaminetetraacetic acid
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Ethylenediaminetetraacetic Acid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris edta buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tris edta buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Edta Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pellet tris low edta suspension buffer  (Teknova)
94
Teknova pellet tris low edta suspension buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Pellet Tris Low Edta Suspension Buffer, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris  (Chem Impex International)
96
Chem Impex International tris
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egg yolk tris buffer glycerol solution  (Eppendorf AG)
99
Eppendorf AG egg yolk tris buffer glycerol solution
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Egg Yolk Tris Buffer Glycerol Solution, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spheroplasting media without lys  (AMS Biotechnology)
96
AMS Biotechnology spheroplasting media without lys
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Spheroplasting Media Without Lys, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris–edta buffer  (Fluka Chemical)
90
Fluka Chemical tris–edta buffer
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris–Edta Buffer, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris-hcl buffer solution  (Feiyang Biotechnology Co Ltd)
90
Feiyang Biotechnology Co Ltd tris-hcl buffer solution
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris Hcl Buffer Solution, supplied by Feiyang Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris–hcl buffer (ph 7.5  (Nacalai)
90
Nacalai tris–hcl buffer (ph 7.5
Northern blot analyses of <t>intracellular</t> <t>aminoacylation</t> of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM <t>Tris,</t> pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.
Tris–Hcl Buffer (Ph 7.5, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Journal: ACS synthetic biology

Article Title: Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

doi: 10.1021/acssynbio.5b00197

Figure Lengend Snippet: Northern blot analyses of intracellular aminoacylation of tRNAPyl isoacceptors. Total RNA was obtained from E. coli cells grown in the presence or absence of 3-I-Phe (1 mM) expressing IFRS and (A) either one or five gene copies of tRNAPylACU or (B) one gene copy of tRNAPylCAG, from the pCAM plasmid. Total RNA was suspended in either 10 mM sodium acetate, pH 4.5 (acylated), or 200 mM Tris, pH 8.0 (deacylated, OH−). Positions of acylated and deacylated tRNAPyl isoacceptors are indicated.

Article Snippet: Biochemical Characterization of the IFRS/tRNA Pyl Pair The aminoacylation assays were carried out for 60 min at 37 °C in aminoacylation buffer containing 100 mM Tris-HCl (pH 7.0), 10 mM MgCl 2 , 50 mM potassium chloride, and 5 mM DTT, with 1 mM 3-I-Phe (ChemImpex), 2 mM ATP, 500 nM IFRS, and 5 µM 32 P 3′-end-labeled transcript.

Techniques: Northern Blot, Expressing, Plasmid Preparation